Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans.

Jiangyao Xu,Gaixia Xu,Yushu Wang,Tianying Zhan,Kan Liu,Yang Sun,Xiaoyun Zhang,Wen Liu,Zijun Ouyang,Xiaomei Wang,Tingting Chen

doi:10.18632/aging.102466

Abstract

The biological effects of magnetic fields are a research hotspot in the field of biomedical engineering. In this study, we further investigated the effects of a rotating magnetic field (RMF; 0.2 T, 4 Hz) on the growth of human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans. The results showed that RMF exposure prolonged the lifespan of C. elegans and slowed the aging of HUVECs. RMF treatment of HUVECs showed that activation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) was associated with decreased mitochondrial membrane potential (MMP) due to increased intracellular Ca2+ concentrations induced by endoplasmic reticulum stress in anti-aging mechanisms. RMF also promoted the health status of C. elegans by improving activity, reducing age-related pigment accumulation, delaying Aβ-induced paralysis and increasing resistance to heat and oxidative stress. The prolonged lifespan of C. elegans was associated with decreased levels of daf-16 which related to the insulin/insulin-like growth factor signaling pathway (IIS) activity and reactive oxygen species (ROS), whereas the heat shock transcription factor-1 (hsf-1) pathway was not involved. Moreover, the level of autophagy was increased after RMF treatment. These findings expand our understanding of the potential mechanisms by which RMF treatment prolongs lifespan.

Highlights

The biological effects of exposure to magnetic fields have been widely debated [1,2,3]
Western blot analysis showed that rotating magnetic field (RMF) exposure increased adenosine 5′-monophosphateactivated protein kinase (AMPK) protein expression, and decreased expression of P21, P53 and mTOR proteins in a timedependent manner (Figure 2E)
The results indicated that RMF delays the senescence of human umbilical vein www.aging-us.com endothelial cell (HUVEC) by upregulating signaling via the AMPK pathway and downregulating the mTOR pathway

Summary

Introduction

The biological effects of exposure to magnetic fields have been widely debated [1,2,3]. The effects magnetic fields on cell function and metabolism have been extensively investigated in many areas such as cancer therapy [4], bone development [5], and electrophysiology [6]. Because the direction of the rotating magnetic field (RMF) changes constantly with the rotation of the magnet, it has certain representativeness in the study of the biological effect of magnetic field [7]. The changes in protein structure induced by magnetic fields and the electrophysiological function of magnetic www.aging-us.com fields are the most controversial topics in biophysics [8,9,10,11]. In 2016, the Xie group published the first report of a hypothetical magnetic receptor (MagR) with a unique structure consisting of a multimeric magnetic induction rod-like protein complex. [18] the underlying mechanism by which magnetic fields influence life is unclear [19]

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