Abstract
Objective: To observe the effect of rosuvastatin calcium on the vascular development of zebrafish,and investigate the inhibition mechanism involved.Methods: The zebrafish embryos incubated with rosuvastatin calcium were identified as the experimental group.Meanwhile,the embryos immersed in 0.5% dimethyl sulfoxide(DMSO) were identified as the control group.The embryos were treated with drugs at 0.5 hour post fertilization(hpf) stage of development.After 24 hpf of exposure to the drugs,the numbers of intersegmental vessels of embryos were inspected and recorded by fluorescence microscope.Total RNAs were extracted from 0,6,12 and 24 hpf zebrafish embryos.The expression levels of vascular endothelial growth factor A(VEGF-A) were examined by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR).Results:The growth of intersegmental vessels of zebrafish was significantly inhibited by rosuvastatin calcium.And the inhibition effect of rosuvastatin calcium was dose-dependent in the concentration range of 0.01-0.08 nmol/L.Compared with that of the control group,the expression levels of VEGF-A was reduced in the experiment group,and it was significantly different at 24 hpf(P0.05).Conclusion:Rosuvastatin calcium could inhibit the vascular development of zebrafish by down regulating the expression levels of VEGF-A.
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