Abstract
BackgroundIn vitro maturation (IVM) of oocytes has been widely used in the field of assisted reproductive technology. However, oocytes can be injured by oxidative stress during the process of IVM.MethodsThe present study was designed to evaluate the influences of rosmarinic acid (RA) on the IVM of porcine oocytes and the subsequent development of early-stage embryos as well as its underlying mechanisms. Various concentrations of RA (5 µM, 10 µM, and 25 µM) were treated with porcine oocyte maturation medium during the period of IVM.Results and DiscussionThe results showed that 5 µM RA treatment during the period of porcine oocyte IVM improves blastocyst quality and hatching ability after parthenogenetic activation. Furthermore, the presence of RA during the period of IVM dramatically improved the total number of cells after somatic cell nuclear transfer compared to the number of cells in the control group. Notably, RA treatment during the period of porcine oocyte IVM decreased intracellular reactive oxygen species generation not only in oocytes but also in cumulus cells. Further analysis showed that the intracellular free thiols levels in the oocytes were enhanced by treatment with RA during the period of porcine oocyte IVM compared to the free thiols levels in the control groups. These results indicate that RA improves the developmental competence of porcine oocytes during the IVM period by attenuating oxidative stress.
Highlights
In vitro maturation (IVM) of oocytes refers to the process in which immature oocytes obtained from ovaries are cultured under the appropriate conditions in vitro so that theyHow to cite this article Zhang Y, Guo J, Nie XW, Li ZY, Wang YM, Liang S, Li S. 2019
Cumulus-oocyte complexes (COCs) were aspirated from 3–6 mm antral follicles, and those showing a multilayer of compact cumulus cells and homogeneous ooplasm were collected using Tyrode’s lactate–hydroxyethylpiperazine ethane sulfonic acid (HEPES) medium with 0.1% polyvinyl alcohol (PVA, w/v) and 0.05 g/L gentamycin under a stereomicroscope
The results revealed that treatment with 5 μM rosmarinic acid (RA) during the IVM period significantly increased the rate of blastocyst hatching compared to that of the control group (16.83 ± 1.72% vs. 13.67 ± 2.01%; p < 0.05)
Summary
In vitro maturation (IVM) of oocytes refers to the process in which immature oocytes obtained from ovaries are cultured under the appropriate conditions in vitro so that theyHow to cite this article Zhang Y, Guo J, Nie XW, Li ZY, Wang YM, Liang S, Li S. 2019. Rosmarinic acid treatment during porcine oocyte maturation attenuates oxidative stress and improves subsequent embryo development in vitro. Under oocyte in vitro culture conditions, various types of antioxidants have been added as supplements either alone or in different combinations to attenuate oxidative stress, improving oocyte quality (Liang et al, 2018; Wu et al, 2011; Xu et al, 2018; Yu et al, 2018). Further analysis showed that the intracellular free thiols levels in the oocytes were enhanced by treatment with RA during the period of porcine oocyte IVM compared to the free thiols levels in the control groups. These results indicate that RA improves the developmental competence of porcine oocytes during the IVM period by attenuating oxidative stress
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
