Abstract
Plant cell cultures provide an alternative means for producing secondary compounds in food, cosmetic and pharmaceutical industries. Ehretia asperula Zollinger & Moritzi is used as a traditional medicine for the treatment of liver detoxification, ulcers, tumors, inflammation and enhancing the body's resistance in Vietnam. The study was carried out to select suitable callus line for cell suspension cultures of E. asperula Zollinger & Moritzi and investigate the effects of inoculum size, rotation speed and naphthalene acetic acid (NAA) on the proliferation of cell suspension cultures. In addition, the influence of light intensity on the growth and rosmarinic acid (RA) biosynthesis of cell suspension was also surveyed. After 4 weeks of culture, the white to pale yellow friable callus expanded significantly with a fresh weight (FW) of 0.788 g and a high RA content of 2.062 mg/g FW. An appropriate medium for cell proliferation was the liquid B5 medium, which contained 30 g/l glucose, 0.1 mg/l benzyl adenine (BA) and 0.4 mg/l NAA. The results also demonstrated that a 1:20 ratio (w/v) inoculum size, darkness and rotation speed of 90 rpm were the optimal conditions for the proliferation and RA accumulation to 188.217 mg/l in 4 weeks of culture. These findings showed that E. asperula Zollinger & Moritzi cell suspension cultures could be a potential alternative approach for RA production in vitro.
Highlights
Plant cell cultures may be a viable alternative for obtaining valuable secondary substances while avoiding the restrictions of traditional growing methods (1)
Ehretia asperula Zollinger & Moritzi (8) belongs to the Boraginaceae family, called “Xa Den” with the synonym Celastrus hindsii Benth
The rosmarinic acid (RA) concentration of the W was 2.062 mg/g fresh weight (FW), which is much higher than the RA concentration of the Y
Summary
Plant cell cultures may be a viable alternative for obtaining valuable secondary substances while avoiding the restrictions of traditional growing methods (1). The cultures in a bioreactor have been used to produce secondary metabolites in a variety of medicinal species, including paclitaxel in Taxus (2), shikonin in Lithospermum erythrorhizon (3), steroidal glycosides in Dioscorea deltoidea (4) and RA in Satureja khuzistanica (5). Rosmarinic acid (RA) is a bioactive compound significantly found in the Boraginaceae and Lamiaceae families. RA is undergoing research and application in the field of food technology and medicine (6, 7). Ehretia asperula Zollinger & Moritzi (8) belongs to the Boraginaceae family, called “Xa Den” with the synonym Celastrus hindsii Benth
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