Rosmarinic acid improves hypertension and skeletal muscle glucose transport in angiotensin II-treated rats

Mujalin Prasannarong,Yupaporn Rattanavichit,Jariya Buniam,Natsasi Chukijrungroat,Juthamard Surapongchai,Vitoon Saengsirisuwan

doi:10.1186/s12906-019-2579-4

Abstract

BackgroundRosmarinic acid (RA) is a natural pure compound from herbs belonging to the Lamiaceae family, such as rosemary, sage, basil, and mint. The antioxidant, angiotensin-converting enzyme inhibitory, and vasodilatory effects of RA have been revealed. Angiotensin II (ANG II) is a potent agent that generates hypertension and oxidative stress. Hypertension and skeletal muscle insulin resistance are strongly related. The aim of this study was to evaluate the effects of acute and chronic RA treatment on blood pressure and skeletal muscle glucose transport in ANG II-induced hypertensive rats.MethodsEight-week-old male Sprague Dawley rats were separated into SHAM and ANG II-infused (250 ng/kg/min) groups. ANG II rats were treated with or without acute or chronic RA at 10, 20, or 40 mg/kg. At the end of the experiment, body weight, liver and heart weights, oral glucose tolerance, skeletal muscle glucose transport activity, and signaling proteins were evaluated.ResultsBoth acute and chronic RA treatment decreased systolic, diastolic, and mean arterial blood pressure. Only acute RA at 40 mg/kg resulted in a reduction of fasting plasma glucose levels and an induction of skeletal muscle glucose transport activity. These effects might involve increased ERK activity in skeletal muscle. Meanwhile, chronic RA treatment with 10, 20, and 40 mg/kg prevented ANG II-induced hyperglycemia.ConclusionsBoth acute and chronic RA treatment attenuated ANG II-induced cardiometabolic abnormalities in rats. Therefore, RA would be an alternative strategy for improving skeletal muscle glucose transport and protecting against ANG II-induced hypertension and hyperglycemia.

Highlights

Rosmarinic acid (RA) is a natural pure compound from herbs belonging to the Lamiaceae family, such as rosemary, sage, basil, and mint
The final body weights of the ANG Angiotensin II (II) rats were significantly reduced compared with the SHAM rats (Table 1 and Table 2)
At the end of the experiment, the liver weight to body weight ratio was not significantly changed, whereas the heart weight to body weight ratio increased by 0.77–0.95 g/kg (Table 1 and Table 2; P < 0.05)

Summary

Introduction

Rosmarinic acid (RA) is a natural pure compound from herbs belonging to the Lamiaceae family, such as rosemary, sage, basil, and mint. The aim of this study was to evaluate the effects of acute and chronic RA treatment on blood pressure and skeletal muscle glucose transport in ANG II-induced hypertensive rats. Rosmarinic acid (RA) is a natural pure compound from herbs that belong to the Lamiaceae family, such as rosemary, sage, basil, and mint. These plants are widely and routinely used in cooking recipes. Angiotensin II (ANG II) is a potent hypertensive agent It is involved in the generation of reactive oxygen species (ROS) that activate p38 MAPK, decrease Akt phosphorylation, and decrease GLUT-4 translocation in skeletal muscles

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