Abstract
Rosette formation of guinea pig thymocytes (Th) and thymus-derived peripheral blood lymphocytes (TBL) was tested under different experimental conditions. Up to 93% of Th and 38% of TBL showed an affinity to rabbit red blood cells (RRBC). Treatment with metabolic inhibitors like sodium azide and sodium cyanide or freezing and thawing nearly abolished rosette formation by TBL but was ineffective with respect to Th. Heating the cells destroyed rosette-forming capacity of both cell types. These results indicate that spontaneous rosette formation with RRBC by Th does not require the live cell.
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