Roseovarius confluentis sp. nov., isolated from estuary sediment.

Abstract

A Gram-stain-negative and strictly aerobic bacterium, designated strain SAG6T, was isolated from estuary sediment in South Korea. Cells of strain SAG6T were found to be oxidase- and catalase-positive rods with gliding motility. Cell growth was observed at 15-40 °C (optimum 30 °C), at pH 6.5-10.0 (optimum pH 7.0) and in the presence of 0.5-13.0 % (w/v) NaCl (optimum 2.0 %). Ubiquinone-10 was the only detected respiratory quinone and summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), C16 : 0, C18 : 1ω7c 11-methyl and C12 : 0 were the major fatty acids identified (>5 % of the total fatty acids). The polar lipids of strain SAG6T consisted of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid and two unidentified lipids. The G+C content of the genomic DNA was 66.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SAG6T formed a tight phyletic lineage within the genus Roseovarius. Strain SAG6T was most closely related to Roseovarius indicus B108T with 97.6 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and molecular features, strain SAG6T clearly represents a novel species of the genus Roseovarius, for which the name Roseovarius confluentis sp. nov. is proposed. The type strain is SAG6T (=KACC 18598T=JCM 31541T).