Roseovarius carneus sp. nov., a novel bacterium isolated from a coastal phytoplankton bloom in Xiamen.

Abstract

A Gram-stain-negative, non-motile, ovoid or short rod shaped and aerobic marine bacterium, designated as strain LXJ103T, was isolated from a coastal phytoplankton bloom in Xiamen, PR China. Cells were oxidase- and catalase-positive. Strain LXJ103T grew at 4-40 °C (optimum, 28-37 °C), at pH 6-10 (optimum, pH 8.5) and with 1-15 % (w/v) NaCl (optimum, 3 %). The major cellular fatty acids (>10 %) were iso-C18 : 1 ω7c/iso-C18 : 1 ω6c (70.2 %) and C16 : 0 (10.3 %). The following polar lipids were found to be present: phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and five unknown glycolipids. The predominant respiratory quinone was ubiquinone-10. Strain LXJ103T exhibited the highest 16S rRNA gene sequence similarity to Roseovarius litorisediminis D1-W8T (96.97 %). The phylogenetic trees based on 16S rRNA gene sequences showed that strain LXJ103T was a member of the genus Roseovarius. The draft genome size of strain LXJ103T is 3.05 Mb with a genomic G+C content of 61.22 mol%. The digital DNA-DNA genome hybridization value of strain LXJ103T compared with the most similar type strain R. litorisediminis CECT 8287T was 18.80 %. The average nucleotide identity value between strain LXJ103T and R. litorisediminis CECT 8287T was 72.60 %. On the basis of polyphasic data, strain LXJ103T represents a novel species of the genus Roseovarius, for which the name Roseovarius carneus sp. nov. is proposed. The type strain is LXJ103T (=CGMCC 1.19168T=MCCC 1K06527T=JCM 34778T).