Abstract
Two Gram-negative, aerobic, rod-shaped and yellow-orange pigmented bacterial strains (LMG 31523T and LMG 31524) were isolated from roots of wild-growing Alkanna tinctoria plants collected near Thessaloniki, Greece. Analysis of their 16S rRNA gene sequences revealed that they form a separate cluster related to the genus Roseomonas. A comparative whole genome analysis of the two strains and the type strains of related Roseomonas species revealed average nucleotide identity values from 78.84 and 80.32%. The G + C contents of the genomic DNA of strains LMG 31523T and LMG 31524 were 69.69% and 69.74%, respectively. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that the strains LMG 31523T and LMG 31524 represent a novel species of the genus Roseomonas. Genome analysis of the new strains showed a number of genes involved in survival in the rhizosphere environment and in plant colonization and confirmed the endophytic characteristics of LMG 31523T and LMG 31524. Since the strains LMG 31523T and LMG 31524 were isolated from a plant collected in Greece the name Roseomonas hellenica sp. nov. is proposed. The type strain is LMG 31523T (=CECT 30032T).
Highlights
The first Roseomonas strains were isolated by Gilardi and Faur [16] and described as relatives of Methylobacterium
While the first species originated from clinical samples, more recently bacteria from the genus Roseomonas have been reported from various other environments, including air [49], soil [12,27,37,50], activated sludge [32], lake sediments [24], freshwater and drinking water [2,15], and plants [11,36]
The phylogenetic comparison of the nearly complete 16S rRNA gene sequences of LMG 31523T and LMG 31524 with data from EzTaxon revealed that the strains are related to the genus Roseomonas with a similarity of 99.92% to each other
Summary
The first Roseomonas strains were isolated by Gilardi and Faur [16] and described as relatives of Methylobacterium. The type strains of related Roseomonas species for which no genomic sequence was available were sequenced (i.e., R. arctica LMG 28251T, R. eburnea LMG 31228T, R. oryzicola LMG 31161T, R. alkalliterrae LMG 31230T, R. soli LMG 31231T, R. terrae LMG 31159T).
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