Rosanortriterpenes A-B, Two Promising Agents from Rosa laevigata var. leiocapus, Alleviate Inflammatory Responses and Liver Fibrosis in In Vitro Cell Models.

Bai-Lin Li,Juan-Juan Hu,Jie-Wei Wu,Jie Yuan,Chen Ni,Qian-Ran Li,Jin-Dan Xie,Hui-Jun Liang,Jianbo Wan

doi:10.1155/2020/8872945

Abstract

Rosanortriterpenes A–B (RTA and RTB), two nortriterpenoids, are characteristic constituents in the fruits of Rosa laevigata var. leiocapus. However, pharmacological studies on these compounds are still scarce. In the present study, we aim to investigate the anti-inflammatory mechanisms associated with the effects of RTA–B in RAW264.7 macrophages and LO2 cells by detecting cell viabilities, nitric oxide (NO) production, tumour necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) production. Simultaneously, the anti-inflammatory action mechanisms of these two compounds were illustrated through western blot assay. Besides, the antihepatic fibrosis activities of these compounds have also been explored. The results demonstrated that RTA and RTB inhibited the production of NO, TNF-α, and IL-6 and suppressed liver fibrosis. RTA and RTB treatment also greatly inhibited the activation of NF-kappaB (NF-κB) pathway. Our study confirmed the promising anti-inflammatory and anti-liver fibrosis actions of RTA–B, suggesting that they might be developed as alternative and promising drugs for the treatment of hepatic inflammatory and fibrotic diseases.

Highlights

Inflammation is an immune response caused by tissue damages or pathogen infections [1]
Even at the concentration of 100 μM, viabilities of the two cells treated with both RTA–B were basically unchanged compared to the blank control group (Figures 2(a) and 2(b)), suggesting cytosafety of these compounds
We investigated the cell viability of LPS-stimulated RAW264.7 treated with RTA and RTB at the concentration of 3, 10, and 30 μM with 10 μM Indo acting as a positive control. e results are shown in Figure 2(c). 100 ng/mL LPS could stimulate the proliferation of RAW264.7; 24 h treatment by RTA and RTB could reduce the proliferation in a dose-dependent manner

Summary

Introduction

Inflammation is an immune response caused by tissue damages or pathogen infections [1]. Excessive and uncontrolled inflammations are harmful to the body, especially liver, the largest metabolic organ in the human body, as they may cause chronic diseases and further lead to fatty liver diseases, hepatitis, liver fibrosis, and even cirrhosis and liver cancer [2,3,4,5,6]. Traditional diets, in particular, edible plants, with pharmacological functions such as pigeon pea, orange peel, and grapefruit [13,14,15] have been always regarded as an essential source for treatment of inflammation. Erefore, it may be a potential way to seek new anti-inflammatory agents from traditional medicinal food [16]. Its fruits are recorded in Chinese Pharmacopoeia as a treatment for spermatorrhea, uroclepsia, frequent micturition, and uterine abscission and considered as a kind of traditional

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