Abstract
The dye Rosaniline hydrochloride (RANH) has been successfully incorporated in MCM-48 (designated as RANH@MCM-48) and characterized by various spectroscopic methods including FT-IR, SEM, EDX and N2 adsorption-desorption isotherm. RANH@MCM-48 in aqueous medium acts as fluorescence "on" sensor for neurotransmitter dopamine (DA) in presence of its main biological interfering agent ascorbic acid or vitamin c (AA) along with Glucose, Cholesterol and Uric acid (UA). The limits of detection (LOD) were found to be 65nM and 51nM respectively in absence and in presence of AA. The interaction of DA to RANH@MCM-48 is found to be reversible with respect to EDTA2-. The fluorescence intensity vs. pH plot shows a narrow fluorescence window of 7.2 to 8.8. RANH@MCM-48 has been successfully applied for DA detection in artificial cerebrospinal fluid (ACF) and bovine serum albumin (BSA) with LOD values 27nM and 22.5nM respectively. Platinum disc electrode has been modified with RANH@MCM-48 which showed distinct oxidation peaks with a separation of 0.188V in cyclic voltammetry (CV). The LOD for DA in presence of AA determined from oxidation current is 77.5nM. The voltammetric detection of DA is found to be free from common interfering species Na+, K+, Ca2+, Fe2+, UA, Cholesterol and Glucose. RANH@MCM-48 has been found to be a very effective fluorescence and voltammetric sensor for DA with very low LOD.
Highlights
Dopamine (DA) is a widely studied neurotransmitter belonging to catecholamine family which plays essential roles in the functioning of central nervous system, cardiovascular system, hormonal system, human behavioural responses such as mood, emotion, memory, and addiction [1,2,3]
Rosaniline hydrochloride (RANH)@MCM-48 has been successfully applied for DA detection in artificial cerebrospinal fluid (ACF) and bovine serum albumin (BSA) with limits of detection (LOD) values 27 nM and 22.5 nM respectively
We report that Rosaniline Hydrochloride dye encapsulated in MCM-48 (RAN@MCM-48) acts as a fluorescent sensor for the neurotransmitter dopamine in presence of biomolecules which commonly coexists with dopamine in biological fluids
Summary
Dopamine (DA) is a widely studied neurotransmitter belonging to catecholamine family which plays essential roles in the functioning of central nervous system, cardiovascular system, hormonal system, human behavioural responses such as mood, emotion, memory, and addiction [1,2,3]. RANH@MCM-48 in aqueous medium acts as fluorescence “on” sensor for neurotransmitter dopamine (DA) in presence of its main biological interfering agents ascorbic acid or vitamin c (AA) along with Glucose, Cholesterol and Uric acid.
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