Abstract
The role of ROS in stem cell biology has not been fully illustrated and understood. Here we compared the different responses and investigated the mechanism underlying oxidative stress induced by hydrogen peroxide (H2O2) between murine corneal epithelial progenitor cell line (TKE2) and mature murine corneal epithelial cells (MCE). TKE2 showed a different homeostasis and strong resistance to H2O2. TKE2 reduced the production of ROS, inhibited ROS generation enzyme NADPH oxidase 4 (NOX4), and increased dual specificity phosphatase 6 (DUSP6). Furthermore, TKE2 activated nuclear factor (erythroid-derived 2)-like 2 (NRF2) signaling pathway, regulated miR-125B1 and miR-29B1, and elevated levels of antioxidants glutathione S-transferase P (GSTP) and superoxide dismutases (SOD). The association with ROS of the cells was also verified by RNA interference approach and pharmacological antagonization. In addition, TKE2 enhanced the autophagy after exposure to H2O2. The novel evidence suggests that TKE2 cells have different homeostasis and strong antioxidant properties against oxidative stress via the regulation of ROS formation and pathway.
Highlights
The research on complex cell properties and sophisticated cellular functions of stem cells from different origins has drawn increasing attention in the field, due to emerging application and transplantation of stem cells
We compared the different responses of TKE2 cells and mature murine corneal epithelial cells (MCE) to oxidative stress induced by hydrogen peroxide, and investigated the underlying mechanistic by focusing on the association with the reactive oxygen species (ROS) system and signaling pathways
The blots were run under the same experimental conditions and the images were from the same gel
Summary
The research on complex cell properties and sophisticated cellular functions of stem cells from different origins has drawn increasing attention in the field, due to emerging application and transplantation of stem cells. The role of reactive oxygen species (ROS) in stem cell biology has become another important research topic of stem cell properties. The mechanism underlying the role of ROS in stem cell biology remains largely unknown, multiple reports demonstrate that stem cells have antioxidant properties[4,5,6,7], and that oxidative stress status in the environment of stem cells may affect the cellular functions of transplanted stem cells[8,9,10,11]. We compared the different responses of TKE2 cells and mature murine corneal epithelial cells (MCE) to oxidative stress induced by hydrogen peroxide, and investigated the underlying mechanistic by focusing on the association with the ROS system and signaling pathways
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