Abstract
Objective: Here we describe a study where we compare the cell characteristics and differentiation potential of porcine bone marrow derived mesenchymal stem cells cultured under hypoxic (5% O2) and normoxic (20% O2) conditions. Methods and Results: Porcine bone marrow was used to derive mesenchymal stem cells using human platelet lysate cell culture supplement under both hypoxic (5% O2) and normoxic (20% O2) conditions, flow cytometry and differentiation was used to characterize the cell populations. Cells cultured under normoxic conditions differentiated at a greater rate compared to cells cultured 100% of the time in severe hypoxia. Differentiation potential of hypoxic cultured cells was increased when cells were transferred into normoxia. Conclusions: In our experimental conditions, severe hypoxia (100% hypoxia) influenced the ability of porcine bone marrow derived stem cells to differentiate. Adipogenic, osteogenic, and chondrogenic differentiations are decreased in severe hypoxic conditions, but increased when transferred to normoxic conditions under differentiation. Our study is the first to compare the stem cell characteristics of hypoxic and normoxic porcine derived bone marrow mesenchymal stem cells.
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