Root-knot nematodes (Meloidogyne hapla) can modify the positive plant intraspecific diversity-productivity effect on red clover in clover-grass communities

Abstract

Plant diversity and soil organisms are both widely studied for their contributions to ecosystem functions. It has been recently shown that soil biota may drive the plant species diversity-function relationship in grasslands, while so far studies on plant intraspecific diversity are lacking. To improve the understanding of how ecosystem functions may be influenced by plant intraspecific diversity, soil organisms, and their interactions, we used a field setting to examine the effects of plant intraspecific diversity and functionally dissimilar soil organisms (manipulated by the addition of northern root-knot nematodes, earthworms, and arbuscular mycorrhizal fungi) on aboveground primary production, pollination and shoot herbivory. Plant intraspecific diversity was manipulated by sowing different seed mixtures of Trifolium pratense L. and Lolium perenne L. cultivars. We found a positive effect of plant intraspecific diversity on total aboveground primary production, contributed mostly by T. pratense. The plant intraspecific diversity-productivity effect on T. pratense was mediated by the nematodes, i.e. aboveground biomass was increased by intraspecific diversity only in the nematode treatment. Further, the average floral visitation length was decreased by earthworm addition, while the abundance of pollinators tended to be increased by high plant intraspecific diversity. Leaf damage of T. pratense were not influenced by the treatments. We conclude that plant intraspecific diversity and soil organisms can interactively or independently modify functions such as aboveground primary production and pollination of T. pratense in the field. Specifically, the positive plant intraspecific diversity effect appears to only emerge under conditions of stress (high nematode abundance). This result indicates that plant intraspecific diversity may be important for the maintenance of ecosystem functions, especially in an agricultural context, where pest pressure can be high and can have large detrimental consequences.