Abstract
Method of rooting in petioles was developed for leaves of Arachis plants. The objective was the development of methodology that allows the chromosome number determination for evaluation of the ploidy level in colchicine-treated branches. In order to obtain a high frequency of metaphases and chromosomes with clear morphology, roots were pretreated with 8-hydroxyquinoline combined to cycloheximide for 2 h. Root tips were stained using Feulgen dye for chromosome analysis. This method can be applied also for cytological studies of materials in which seeds are not available or cutting is not feasible.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
