Root water uptake under heterogeneous soil moisture conditions: an experimental study for unraveling compensatory root water uptake and hydraulic redistribution

Abstract

Plant roots often encounter heterogeneity in soil water content and respond by compensating water uptake from wet zones to cope with the transpiration demand. Simultaneously, plants may also exhibit root-mediated hydraulic redistribution from wet to dry zones. Experiments were conducted to simultaneously monitor compensated root water uptake and hydraulic redistribution in the vadose zone. Vertical and horizontal split-root lysimeters were used to hydraulically isolate maize roots under altering soil water conditions. Compensated root water uptake and root-mediated hydraulic redistribution were monitored by continuous measurement of water content in the lysimeter compartments. Soil water heterogeneity and limited soil water availability were found to accelerate the root water uptake from moist region to compensate the reduced water availability in dry zone. However, no measurable root mediated hydraulic redistribution was observed in short term basis, despite high water potential gradient in the root zone of both lysimeters. The night-time transpiration and xylem refilling processes seem to override the hydraulic redistribution on a diurnal basis in our experiment. Our study shows that compensated root water uptakeplays a major role in meeting the transpiration demand. In contrast, the role of root mediated hydraulic redistribution is found to benegligible in maize plant.