Abstract
Summary ROOT HAIR DEFECTIVE SIX‐LIKE4 (RSL4) is necessary and sufficient for root hair elongation in Arabidopsis thaliana. Root hair length is determined by the duration for which RSL4 protein is present in the developing root hair. The aim of this research was to identify genes regulated by RSL4 that affect root hair growth.To identify genes regulated by RSL4, we identified genes whose expression was elevated by induction of RSL4 activity in the presence of an inhibitor of translation.Thirty‐four genes were identified as putative targets of RSL transcriptional regulation, and the results suggest that the activities of SUPPRESSOR OF ACTIN (SAC1), EXOCSYT SUBUNIT 70A1 (EXO70A1), PEROXIDASE7 (PRX7) and CALCIUM‐DEPENDENT PROTEIN KINASE11 (CPK11) are required for root hair elongation.These data indicate that RSL4 controls cell growth by controlling the expression of genes encoding proteins involved in cell signalling, cell wall modification and secretion.
Highlights
Root hairs are tubular projections that develop from the outermost layer of roots
We hypothesized that the immediate targets of ROOT HAIR DEFECTIVE SIX-LIKE4 (RSL4) would be required for root hair elongation
To identify downstream targets of RSL4, we developed an inducible system by expressing a glucocorticoid receptor:RSL4 fusion protein (GR: RSL4) in the root hairless rsl2 rsl4 double mutant background (Lloyd et al, 1994)
Summary
Root hairs are tubular projections that develop from the outermost layer of roots (epidermis or periderm). Root hairs are longer in roots grown in low-phosphate conditions than when grown in replete phosphate conditions This adaptive response to low phosphate availability increases the capacity for phosphate uptake because longer hairs can mine larger volumes of the soil for phosphate (Brown et al, 2013; Lopez-Arredondo et al, 2014). Such phenotypic plasticity is characteristic of plant development and is often under strict transcriptional control (Rubio et al, 2001; Jain et al, 2012)
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