Root exuded low-molecular-weight organic acids affected the phenanthrene degrader differently: A multi-omics study

Abstract

As a class of highly toxic and persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) are an increasingly urgent environmental problem. Low-molecular-weight organic acids (LMWOAs) are important factors that regulate the degradation of PAHs by plant rhizosphere microorganisms, which affect the absorption of PAHs by plant roots. However, the comprehensive mechanisms by which LMWOAs influence the biodegradation of PAHs at cellular and omics levels are still unknown. Here, we systematically analyzed the roles of citric, glutaric and oxalic acid in the PAH-degradation process, and investigated the mechanisms through which these three LMWOAs enhance phenanthrene (PHE) biodegradation by B. subtilis ZL09–26. The results showed that LMWOAs can improve the solubility and biodegradation of PHE, enhance cell growth and activity, and relieve membrane and oxidative stress. Citric acid enhanced PHE biodegradation mainly by improving the strain’s cell proliferation and activity, while glutaric and oxalic acid accelerated PHE biodegradation mainly by improving the expression of enzymes and providing energy for the cells of B. subtilis ZL09–26. This study provides new insights into rhizospheric bioremediation mechanisms, which may enable the development of new biostimulation techniques to improve the bioremediation of PAHs.