Root exudate–assisted phytoremediation of copper and lead contamination using Rumex acetosa L. and Rumex K-1

Abstract

Heavy metal pollutants can be effectively removed from soil through phytoremediation using root exudates. Herein, experiments were conducted to assess the phytoremediation capabilities of Rumex acetosa L. and Rumex K-1 root exudates for copper (Cu) and lead (Pb) contamination. Results indicated that these root exudates effectively adsorbed Cu and Pb. Furthermore, the optimal adsorption conditions of Cu by the root exudates of both plants were as follows: light duration of 36 h, light intensity of 8000 Lx, temperature of 25 °C and CO(NH2)2 concentration of 0 %. Moreover, the optimal adsorption conditions of Pb by Rumex acetosa L. and Rumex K-1 root exudates were light duration of 48 h and 24 h, respectively, light intensity of 8000 Lx, temperature of 25 °C and CO(NH2)2 concentration of 0 %. In addition, the root exudates from both plants enhanced the enrichment and transport of Cu and Pb. Moreover, the root was found to be the main accumulation site of Pb, while the stems and leaves were the main accumulation sites of Cu. With the application of root exudates, plant growth increased, with growth indices in Rumex acetosa L. and Rumex K-1 groups treated with exudates being 1.08–1.81-fold and 1.06–1.9-fold higher, respectively, compared with the untreated ones; physiological indexes showed 1.14–2.62-fold and 1.14–2.71-fold improvements, respectively. Remediation efficiency indexes showed 1.05–1.62-fold and 1.10–1.89-fold improvements, respectively. Rumex acetosa L. and Rumex K-1 exhibited promising potential for the phytoremediation of Cu and Pb, with root exudates playing a critical role in metal adsorption and stabilisation, suggesting their potential for enhancing remediation capabilities. This study sheds light on the mechanisms of root exudate–assisted phytoremediation and provides insights into alleviating heavy metal pollution.