Abstract

This chapter provides protocols for the use of a gnotobiotic sand system to study root colonisation after seed inoculation. The complete experimental setup for a gnotobiotic system to grow plants for 7–14 days in the presence of inoculated bacteria or fungi is described. Subsequently, rhizosphere interactions and the in situ behaviour of inoculated organisms is visualised using autofluorescent proteins or other reporter systems. The behaviour of a good root-colonising Pseudomonas strain in this gnotobiotic system is described in terms of distribution, localisation, and root colonisation strategies as observed by microscopy.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.