Abstract
A unique fluid room-temperature phosphorescence (RTP) method, which is different from the RTP methods developed previously and needs no microscopically ordered medium, is developed. The RTP emission of dansyl chloride (DNS-Cl) and its amino acid derivatives can be induced directly in aqueous system by the addition of thallium nitrate as heavy atom perturber and sodium sulfite as deoxygenator, λ ex λ em = 326 574 nm . A good linearity between the RTP intensity and the concentration of DNS-Cl in the range 2.0 × 10 −7−1.0 × 10 −5 mol/l was obtained with a detection limit of 1.9 × 10 −8 mol/l. A comparative study of RTP of DNS-Cl in the presence of cyclodextrins (CDs), surfactant micelle, organic solvent and bovine serum albumin (BSA) has also been carried out. This fluid RTP emission system of DNS-Cl is very transparent and stable, and can be modulated by different organic solvents. The quenching behavior of RTP by BSA presents a satisfactory linearity between the concentration of BSA and I RTP , which can be used in the quantitative analysis of BSA.
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