Abstract
The formation of porous gold nanostructures in aqueous medium at room temperature using transmetallation reaction (TM reaction) that involves sacrificial silver nanoparticles and Au3+ ions (HAuCl4) controlled using a naturally available chicken egg shell membrane is reported in this article. The formation of porous gold nanostructures via TM reaction is inferred from UV-Vis absorption spectra. Crystallinity is confirmed by X-ray diffraction (XRD) and selected area electron diffraction (SAED) patterns. Porous gold tube like structures seen in the Field Emission Scanning Electron Microscopic (FESEM) images was confirmed by the Transmission Electron Microscopic (TEM) images. Energy dispersive spectroscopy (EDS) confirmed the presence of only gold in the final product. The porous gold nanostructures could show fluorescence in Yeast cells, but the cell intake was limited. Glucose functionalized porous gold nanostructures could enter Yeast cells showing fluorescence, but the cells got killed. The synthesized porous nanostructures tagged with the cell permeant dye acridine orange (AO) was found to increase the fluorescence substantially in cell staining, compared to bare AO and AO tagged solid AuNPs. Present work illustrates the potential use of porous gold nanostructures in bio-imaging.
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