Abstract

Some coumarin derivatives have been used as fluorescence labelling agent. The room temperature phosphorescence (RTP) properties of 27 kinds of coumarin derivatives on filter paper have been studied. It is observed that most of them can give more intensive RTP signals when using 1.0 mol l −1 lead acetate as a heavy-atom perturber and that there is an obvious relation between the RTP characteristics and the substituting group in some of the derivatives. The fact that many of the coumarin derivatives can give RTP emission means that they can be expected to serve as RTP labelling agent.

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