Ronin Is Essential for Embryogenesis and the Pluripotency of Mouse Embryonic Stem Cells

Abstract

(Cell 133, 1162–1174; June 27, 2008) It has come to our attention that some of the lacZ staining patterns representing Ronin expression in the brain are not readily apparent in the original Figure 1D and that the scatter plots depicted in Figure 6A require explanation in the legend. We are therefore providing enlarged images of each of the three brain regions in which Ronin expression was observed, as well as an expanded legend for Figure 6A. Figure 6A. Revised Legend (A, left) Box plots showing results of microarray analysis of control ES cells and ES cells 24 hr after transfection with pEF1α-hRonin-Flag. Each box represents median and 75th and 25th percentile values. (A, right) M-A-plot with M (y axis) calculated as the difference between log intensities of the experimental data set and a pseudo median reference data set. The expression measures for the pseudo median data were calculated by taking, for each probe set, the median log expression for all chips in the study. Ronin-transfected ES cells differ from control ES cells across a broad range of intensities. Ronin Is Essential for Embryogenesis and the Pluripotency of Mouse Embryonic Stem CellsDejosez et al.CellJune 27, 2008In BriefPluripotency is a unique biological state that allows cells to differentiate into any tissue type. Here we describe a candidate pluripotency factor, Ronin, that possesses a THAP domain, which is associated with sequence-specific DNA binding and epigenetic silencing of gene expression. Ronin is expressed primarily during the earliest stages of murine embryonic development, and its deficiency in mice produces periimplantational lethality and defects in the inner cell mass. Conditional knockout of Ronin prevents the growth of ES cells while forced expression of Ronin allows ES cells to proliferate without differentiation under conditions that normally do not promote self-renewal. Full-Text PDF Open Archive