Abstract
Roles of zebrafish Nipped-B-like (Nipbl) in gene regulation
Highlights
Prep1 (Pknox1) is a homeodomain transcription factor in theTALE superfamily and a critical regulator of hematopoiesis and organogenesis
The insertional mouse mutation Acrodysplasia (Adp) confers a parentof-origin developmental phenotype, with animals inheriting the mutation from their father showing skeletal abnormalities, while those inheriting the mutation from their mother are normal
To identify the earliest transcriptional changes due to loss of Nipbl function, microarray analyses were performed on morphants at gastrula stages (6.5–9.0 hpf). When both zNipbls were depleted with translation-blocking MOs, at least 7 genes were significantly up-regulated by 6.5 hpf, just hours after the mid-blastula transition (MBT, onset of zygotic transcription)
Summary
Prep1 (Pknox1) is a homeodomain transcription factor in theTALE superfamily and a critical regulator of hematopoiesis and organogenesis. Title Roles of zebrafish Nipped-B-like (Nipbl) in gene regulation Using protein-binding microarray analysis, we identified two tandem highly conserved Prep1 bindings sites in the Pax6 EE, predicted to be of medium-affinity binding strength. We define a novel cis-regulatory logic, whereby medium-affinity transcription factor binding sites act additively to define a genetic threshold for gene activation.
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