Roles of transforming growth factor-β1 during bone mesenchymal stem cells transplantation for rat hepatic fibrogenesis and hepatoma model

Abstract

Objective To investigate the effects of bone mesenehymal stem cells (BMSCs) transplantation on rat hepatic fibrogenesis/hepatoma via transforming growth factor-β1 (TGF-β1).Methods The hepatic fibrosis model and hepatoma model were induced by carbon tetrachloride (CCl4) and diethylnitrosamine respectively in rats (n =16 each).The BMSCs of the forth generation were respectively injected into the rats via the caudalis vena.Effects of TGF-β1 on the BMSCs transplantation on hepatic fibrogenesis and hepatoma in rats were investigated.Results In the hepatic fibrosis model,the mean value of Scheuer inflammation score,Schmid M fibrosis score and the activation score of HSC showed significant difference between control group and intervention group,with 3.58 and 0.42 (u =-4.22,P ＜ 0.01),5.17 and 1.58 (u =-3.65,P ＜0.01),2.25 and 0.58 (u =-2.93,P ＜0.01),respectively.There was also significant difference in the contents of TGF-β1 between two groups (F =11.024,P ＜ 0.05).In the hepatoma model,the cell cycle was restrained in intervention group and most cells were arrested in G1 phase,and in control group,most cells were arrested in S phase (G1 phase P ＜ 0.01,G2 phase P ＜ 0.01,S phase P ＜0.01).However,there was no significant difference in the incidence of liver cancer between two groups (P ＞ 0.05).Further more,the mean value of TGF-β1 showed no significant difference between two groups (P ＞ 0.05).Conclusion BMSCs transplantations can reduce hepatic stellate cells activation and thus slow down the process of liver fibrosis probably by inhibiting the secretion of TGF-β1.BMSCs can inhibit the cell cycle not through the TGF-β1 pathway. Key words: Bone mesenchymal stem cells; Hepatic fibrogenesis; Transforming growth factor-β1