Roles of Proliferation and Angiogenesis in Locally Aggressive Biologic Behavior of Ameloblastoma versus Ameloblastic Fibroma.

Amr Ibrahim,Kamal Abd El-Rahman,Magdy Alazzazi,Emad Alqalshy,Ahmed Abdel-Shakour Abdel-Hafiz

doi:10.3390/diagnostics12020392

Amr Ibrahim, Kamal Abd El-Rahman + Show 3 more

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https://doi.org/10.3390/diagnostics12020392

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Journal: Diagnostics	Publication Date: Feb 3, 2022
Citations: 1	License type: CC BY 4.0

Affiliation: Minia University, Al Azhar University, Iraqi University

Abstract

(1) Background: The present study was carried out to evaluate the roles of proliferation and angiogenesis in locally aggressive biologic behavior of ameloblastoma versus ameloblastic fibroma; (2) Methods: 30 formalin-fixed paraffin embedded blocks (15 cases of ameloblastoma and 15 cases of ameloblastic fibroma) were used. To evaluate the proliferation, the tissue sections were stained with an AgNORs stain. CD105 was used as an immunohistochemical marker of angiogenesis. Quantitative evaluations of AgNORs were performed. The mean vascular density was evaluated as a measure for CD105 protein expression by using image analyzer computer system; (3) Results: The mean number of AgNORs dots per nucleus was significantly higher in ameloblastoma as compared to ameloblastic fibroma. Additionally, the protein level of CD105 showed positive expression and wide distribution that the mean vascular density was significantly higher in ameloblastoma as compared to ameloblastic fibroma; (4) Conclusion: Quantitative evaluation of the AgNORs stain and the mean vascular density utilizing CD105 protein expression may reflect a higher proliferative activity and a more locally aggressive biologic behavior of ameloblastoma when compared to ameloblastic fibroma, indicating that other factors may be involved in biologic behavior of ameloblastic fibroma.

Highlights

Odontogenic tumors are rare lesions that arise from odontogenesis-related cells and tissues, as well as their remains
Quantitative assessment showed that the mean number of AgNORs dots per nucleus was 1.65 ± 0.24 for ameloblastoma (Table 1), located in the nuclei of peripheral cells and central cells of odontogenic epithelial tumor cells (Figure 1A)
Quantitative assessment showed that the mean number of AgNORs dots per nucleus was 1.12 ± 0.21 for ameloblastic fibroma (Table 1), located in nuclei of peripheral cells and central cells of the neoplastic odontogenic epithelial portion (Figure 1B)

Summary

Introduction

Odontogenic tumors are rare lesions that arise from odontogenesis-related cells and tissues, as well as their remains. These tumors are a diverse category of lesions that include hamartomatous lesions as well as benign and malignant neoplasms [1]. Organization (WHO) classification, which has been refined, categorized the benign odontogenic tumors into those of odontogenic epithelial origin, mixed (Epithelial-Mesenchymal) origin, and mesenchymal origin [1]. Ameloblastoma is a rare, odontogenic tumor derived from odontogenic epithelium. Ameloblastoma represents about 1% of all jaw tumors but is considered to be the second-most common odontogenic tumor, benign, and is known for its locally invasive behavior with a high risk of recurrence [2]. Ameloblastic fibroma is a rare mixed odontogenic tumor which constitutes 2% of odontogenic tumors

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