Abstract
Since the discovery of the DNA-dependent ATPase activity of RecA protein of Escherichia coli (Ogawa et al. 1979; Roberts et al. 1979) and the RecA protein-promoted pairing of homologous DNA molecules (McEntee et al. 1979; Shibata et al. 1979b), various kinds of DNA reactions promoted by the protein have been revealed (for review, see Radding 1982). In vitro at a neutral pH, RecA protein promotes: (1) single-stranded DNA (ssDNA)-dependent ATP hydrolysis; (2) superhelical DNA-dependent ATP hydrolysis; (3) binding to DNA; (4) pairing of double-stranded DNA (dsDNA) and the homologous DNA with single-stranded region(s); (5) reciprocal exchange of DNA strands between two dsDNA molecules; (6) polar elongation of heteroduplex joints; (7) dissociation of D-loops in closed circular dsDNA and inactivation of the DNA (Shibata et al. 1982a); (8) ssDNA-dependent unwinding of the double helix; and (9) ssDNA-independent propagation of unwinding of the double helix (Ohtani et al. 1982b; Iwabuchi et al....
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