Year-end Sale % OFF 
Abstract
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-κB, IFN-γ, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte subsets. Compared with the sham and blank groups, the mRNA and protein expressions of MCP-1 and NF-κB in the experimental group were significantly increased. The experimental group had lower serum levels of IL-2 and IFN-γ and higher serum level of IL-10 than the sham and blank groups. In comparison to the sham and blank groups, CD4+ T lymphocyte subsets percentage, CD4+/CD8+ ratio and CD4+ CD25+ Foxp3+ Tregs subsets accounting for CD4+ lymphocyte in the experimental group were lower, while percentage of CD8+ T lymphocyte subsets was higher. Our study provided evidence that higher expression of MCP-1 and NF-κB may be associated with decreased immune function of spinal tuberculosis, which can provide a new treatment direction for spinal tuberculosis.
Highlights
Spinal tuberculosis, a destructive form of tuberculosis, is very common in young adults and children, it accounts for approximately fifty percent of all cases of musculoskeletal tuberculosis, and its incidence is increasing in developed nations [1]
It is believed that the direct damage caused by a large number of M. tuberculosis in the tissue and the immune-mediated reactions are related to spinal tuberculosis [16]
Understanding the role of the immune response factors involved in spinal tuberculosis will help find new treatment targets for the disease
Summary
A destructive form of tuberculosis, is very common in young adults and children, it accounts for approximately fifty percent of all cases of musculoskeletal tuberculosis, and its incidence is increasing in developed nations [1]. The World Health Organization reported 8.8 million new tuberculosis cases in the world in 2010 [2]. In China, bone and joint tuberculosis are the most common secondary tuberculosis, of which more than 50% are spinal tuberculosis. Changes in the immune system are closely related to the occurrence, development and outcome of tuberculosis, and T cells such as CD4+ and CD8+, which are related to cellular immunity, play important roles. A variety of cytokines released among immune cells can have important impacts on the immune response [4]. Understanding the effect of tuberculosis immune factors is a new direction for studying the treatment of spinal tuberculosis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Brazilian Journal of Medical and Biological Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
