Roles of MiR-101 and its Target Gene Cox-2 in Early Diagnosis of Cervical Cancer in Uygur Women

Chen Lin,Talafu Tuokan,Ya-Jing Zhang,Fei Huang,Gulinaer Kuerban

doi:10.7314/apjcp.2014.15.1.45

Abstract

Early diagnosis is important for cervical cancer treatment. This study aimed to characteriz the microRNA profile and target gene protein levels of cervical cancers in Uygur women for application in early diagnosis. The profiles of miRNA in cervical cancer and chronic cervicitis were analyzed with miRNAmicroarray V4.0. The expression of miR-101 was detected by real-time PCR and locked nucleotide acid in situ hybridization (LNA-ISH). Cox-2 protein levels were assessed by immunohistochemistry. The microarray identified a set of 12 miRNAs significantly decreased in cervical cancer in comparison to the control group. Quantitative RT-PCR analysis showed miR-101 to be significantly downregulated in cancer tissues (p<0.05) while LNA-ISH showed miR-101 positive rates of 80% (20/25) and 8% (5/25) (p<0.05) in the control and cervical cancer groups. Cox-2 positive rates of cervical cancer and control groups were 84% (21/25) and 8% (2/25) (p<0.05). Use of down-regulation of miR-101 and up-regulation of Cox-2 as markers may play a role in early diagnosis of cervical cancer in Uygur women.

Highlights

Cervical cancer is a malignancy with second highest morbidity and mortality in women worldwide
The expression levels of miR-101 were 1.4 ± 0.8 and 3.19 ± 0.9 in cervical cancer and normal cervical tissue (p=0.001) (Table 1).The miR-101 expression pattern was further investigated with locked nucleic acid in situ hybridization (LNA-ISH)
As an aspect of the relationship with the microRNA-101, the prediction based on PicTar and TargetScan DNA

Summary

Introduction

Cervical cancer is a malignancy with second highest morbidity and mortality in women worldwide. Its incidence in developing countries is higher than developed countries (Arbyn et al, 2011). There are 131, 500 new cases each year in China alone. In Xinjiang, especially in the southern border region with poor economic conditions, cervical cancer is one of the leading causes of death in Uygur women with a prevalence rate of 527/100 000 and an early occurrence with the average age of 45.04 years (50.85 years for Han). About 80% cases have progressed to advanced stage when patients come to hospital for treatment (Lalaisuzuke et al, 2006). Prevention, early diagnosis and treatment of cervical cancer increasingly attract attention

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Conclusion