Roles of Hedgehog signaling pathway in adipogenic differentiation potential of porcine adipose-derived mesenchymal stem cells

Caiyun Fan,Yunhai Zhang,Jianbo Cheng,Juhua Wang

doi:10.1590/rbz4720170019

Caiyun Fan, Yunhai Zhang + Show 2 more

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https://doi.org/10.1590/rbz4720170019

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Abstract

ABSTRACT This study examined the effects of purmorphamine and cyclopamine, classical agonists and inhibitors of the Hedgehog (Hh) signaling pathway, in the adipogenic differentiation of porcine adipose-derived mesenchymal stem cells (AMSC) to investigate the roles underlying adipogenic differentiation in AMSC. Porcine-derived AMSC were established, and the Hh signaling pathway was activated or inhibited by treatment with purmorphamine or cyclopamine. The adipogenic differentiation of the porcine AMSC was then analyzed by Oil Red O staining. The expression levels of Hh signaling [...]

Highlights

Adipogenesis is a process by which mesenchymal precursor cells differentiate into adipocytes, which store lipids and serve as a central regulator of metabolism (Rosen et al, 2000; Farmer, 2006; Rosen and MacDougald, 2006)
In the adipose-derived mesenchymal stem cells (AMSC), genes that are involved with Hh signaling, including Smo, Gli1, Gli2, Gli3, Ptc1, Ptc2, transcription factor C/EBP-α, and peroxisome proliferator-activated receptor-γ (PPAR-γ), were detected (Figure 2)
The expression level of the adipocyte characteristic transcription factors C/EBP-α and PPAR-γ gradually increased from day 4 after adipogenic induction

Summary

Introduction

Adipogenesis is a process by which mesenchymal precursor cells differentiate into adipocytes, which store lipids and serve as a central regulator of metabolism (Rosen et al, 2000; Farmer, 2006; Rosen and MacDougald, 2006). Hedgehog signals act early in the adipogenic cascade, upstream of the peroxisome proliferator-activated receptor-γ (PPAR-γ), possibly by altering the potential fates of precursor cells (Suh et al, 2006). Hh signaling alters adipocyte maturation of MSC, whereas it affects only the early differentiation of MSC in rodents (Suh et al, 2006; Fontaine et al, 2008). These results suggest that roles of Hh signaling pathway in the development of fat may be different for individual species

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