Abstract
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
Highlights
As a major hormone in the rat pituitary gland, growth hormone (GH) plays an important role in regulating the growth and metabolism of organisms[1,2,3], and GH production is controlled by central and peripheral signals[4]
Successful expression of hepatic insulin-like growth factor 1 (Igf-1) mRNA is associated with GH[11,12,13]
We identified the interaction between miRNA-543-5p and the Gh1 3’untranslated region (UTR) using a dual luciferase reporter and transfected miRNA-543-5p mimic and inhibitor in a GH3 cell line and rat pituitary primary cells
Summary
As a major hormone in the rat pituitary gland, growth hormone (GH) plays an important role in regulating the growth and metabolism of organisms[1,2,3], and GH production is controlled by central and peripheral signals[4]. The hypothalamus plays a key role in the production and release of GH and regulates GH secretion through GH releasing factors or GH releaseinhibiting factors[5]. It is necessary to control the time and extent of its effect to prevent GH from triggering physiological disorders in organisms[10]. The activity of the GH/IGF-1 axis is considered to be related to age. The finding that individuals over 60 years old have low levels of GH showed that as age increases, endocrine and paracrine Igf-1 provides decreased nutrient
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