Abstract
Biochemical and molecular biological approaches to two anion translocators, a Cl(-)-translocating ATPase and sulfate permease were described for Acetabularia acetabulum, a unicellular marine alga. The primary structures of an almost complete cDNA clone of the 50 kDa subunit and a partial cDNA clone of the 54 kDa subunit of the Cl(-)-ATPase were highly similar to the beta and alpha subunits of the F type ATPase, respectively. A partial cDNA clone encoding the alpha subunit of chloroplast ATPase, and partial cDNA clones coding for the beta subunits of chloroplast and mitochondrial ATPases were also obtained from A. acetabulum. The presence of a small multigene family for the F type ATPases with different ion specificities was strongly suggested for the organism. Sulfate uptake system in this organism was also studied and partial cDNA clones encoding CysA and sulfate binding proteins were obtained. ca. 1.7 kb RNA for cysA gene and 1.55 kb for sbp gene were detected by Northern analysis, respectively. A putative malK gene was also partially cloned.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
