Role of transforming growth factor-β in breast milk for initiation of IgA production in newborn infants

Abstract

Background: Transforming growth factor (TGF)-β has a crucial effect on IgA production, which is the major humoral effector of mucosal immunity. Breast milk contains the abundant amount of TGF-β in the early period of lactation. Aim–study design: To verify the notion that TGF-β in breast milk might contribute to the development of IgA production in newborns, we investigated the association of TGF-β in maternal colostrum with an increase of serum IgA in newborns during the first month of life. Subjects and methods: The concentrations of TGF-β1 and TGF-β2, including IL-6 and IL-10, in colostrum samples from 55 healthy mothers were determined by ELISA. The levels of IgA and IgM in serum samples collected from corresponding newborn babies at birth and at 1 month of age were measured by ELISA. Results: TGF-β1 and TGF-β2 were detected in substantial quantities in all colostrum samples, but IL-6 and IL-10 were present only in a proportion of samples. An increase of serum IgA in newborn during the first month of life was significantly higher than that of serum IgM ( p<0.001). Notably, an increase of serum IgA in newborns during 1 month of life was well correlated with levels of both TGF-β1 ( r=0.38, p=0.005) and TGF-β2 ( r=0.45, p=0.0005) in colostrum, while that of IgM was marginally correlated with colostral TGF-β2 ( r=0.28, p=0.04). The association of increase of serum IgA in newborns with IL-6 and IL-10 in colostrum was not evident. Conclusion: Our findings suggest that TGF-β in colostrum might serve as the starter of IgA production in newborn infants.