Role of the Thyroid Gland in Seasonal Reproduction. II. Thyroxine Allows a Season-Specific Suppression of Gonadotropin Secretion in Sheep*

Abstract

A series of four experiments was conducted to examine the previously described phenomenon that the thyroid gland is required for the breeding season to end in female sheep. Exp 1 tested the hypothesis that the thyroid is required because of its secretion of T4. Ewes thyroidectomized (THX) in the anestrous season either received T4 replacement or no further treatment; seasonal reproductive shifts were compared to those of thyroid-intact controls. All ewes were housed in conditions in which the end of the breeding season results from the expression of an endogenous annual rhythm. The ewes were ovariectomized and given constant-release estradiol (E) implants; reproductive state was assessed from serum LH levels to monitor seasonal shifts in response to E negative feedback. THX did not alter onset of the breeding season (LH rise) but, in the absence of T4 replacement, blocked its end (LH fall). T4 replacement reversed this effect of THX. Exp 2 tested the hypothesis that the thyroid is required only until the onset of the breeding season for reproductive activity to end at its normal time. THX after the breeding season began, however, sustained the elevation in LH, suggesting that the thyroid is required after reproductive onset for the breeding season to end. Exp 3 tested the hypothesis that THX causes a widespread disruption of steroid feedback responses. No effect of THX, however, was observed with respect to either the ability of an E rise to elicit the LH surge or the ability of E, or progesterone, to suppress LH secretion in the breeding season. Of the steroid feedback responses tested, THX altered only the seasonal shift in potency of E negative feedback. Exp 4 examined circulating T4 in thyroid-intact ewes over a 2-yr period. An annual cycle of serum T4 was detected; values reached a peak in winter (late breeding season) and a nadir in summer (late anestrus). Our findings support the concept that the thyroid gland plays a fundamental role in seasonal reproduction in the ewe. In this regard, secretion of T4 after the onset of reproductive activity is required for an endogenously generated change in the neuroendocrine axis that leads to an intensified E negative feedback and an end to the breeding season.