Abstract
The dimensions and refractive properties of erythrocytes and the affinity of their hemoglobin for oxygen have been assessed using laser interference microscopy and Raman spectroscopy. Lowering the pH and addition of 2,3-diphosphoglycerate cause comparable changes in the cell shape and the state of cytoplasm, as well as in the oxygen affinity of hemoporphyrin, but the mechanisms of these effects appear to be different. It is shown that acidification mainly decreases the hemoporphyrin oxygen-binding capacity, whereas 2,3-diphosphoglycerate increases the ability of hemoporphyrin to release oxygen.
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