Role of the serotonergic system in the control of gonadotropin secretion in prepubertal male rats.

Abstract

To analyze the role of brain serotonin in the control of gonadotropin secretion in prepubertal males, serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) concentrations were studied at different ages after administration of 5-hydroxytryptophan (5-HTP), a precursor of serotonin synthesis, alone or in combination with fluoxetine, a specific inhibitor of serotonin uptake; 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH-DPAT), a serotonin 1A agonist; or D,L-p-chlorophenylalanine methyl ester hydrochloride (PCPA), an inhibitor of serotonin synthesis. Also, serum gonadotropin concentrations were measured in castrated males implanted with silastic capsules containing testosterone or 5 alpha-androstane-3 alpha, 17 beta-diol (alpha-diol). We found that: (i) the blockade of the serotoninergic system after PCPA administration did not modify basal gonadotropin secretion but did strongly reduce the response to orchidectomy; (ii) a stimulatory effect of 5-HTP on FSH secretion was only observed in intact males at the age of 8 days; (iii) the serotonin 1A agonist 8-OH-DPAT did not change serum gonadotropin concentrations; (iv) 5-HTP increased serum FSH and LH concentrations in 16-day-old castrated males; (v) FSH and LH serum concentrations decreased after 5-HTP in castrated animals implanted with testosterone, whereas castrated animals implanted with alpha-diol showed, like the intact males, a lack of response to 5-HTP.(ABSTRACT TRUNCATED AT 250 WORDS)