Role of the necrosis factor-κB pathway in dibutyl phthalate mediated effects on human glioma cells

Abstract

The human glioma cell line U251 was incubated for 12 h and 24 h in medium containing dibutyl phthalate at concentrations of 25 µmol/L and 100 µmol/L. Decreased cell viability and increased oxidative stress show the cytotoxicity of dibutyl phthalate. Nuclear factor-κB and nuclear factor-κB pathway-related proteins were altered, which could be attenuated by vitamin E at 20 µmol/L. Thus, dibutyl phthalate induces cytotoxicity through activation of nuclear factor-κB and vitamin E exerts neuroprotection against dibutyl phthalate-mediated cytotoxicity in vitro.