Abstract
Intact plant mitochondria, isolated from climacteric ( Lycopersicon esculentum, Mill., tomato) or non-climacteric ( Solanum tuberosum, L., potato) tissues, and purified on Percoll density gradients, were unable to convert 1-aminocyclopropane 1-carboxylic acid (ACC) to ethylene. Energization or sonication did not enhance ethylene production. For both tissues, the low activity of ACC conversion found in crude mitochondrial fractions from both tissues was increased by sonication. After mitochondrial purification, this activity was located on top of the gradient together with the microsomal membrane fraction containing a high lipoxygenase activity. Addition of exogenous lipoxygenase and linoleic acid to isolated tomato or potato mitochondria greatly enhanced ACC conversion (to approx. 300 pmol h −1 mg −1 protein). Direct measurements of ACC uptake by mitochondria indicated that ACC uptake is not dependent on energization.
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