Abstract
ABSTRACT A strain, 302, of Micrococcus radiodurans mutant in mtcA is very sensitive to the lethal effects of mitomycin C (MTC) and decarbamoyl MTC but is fully UV resistant. Alkaline sucrose gradient analysis of DNA from bacteria treated with 7-bromomethylbenz [ a ]-anthracene (BrMBA) indicates that 302 is unable to initiate excision repair of BrMBA-DNA adducts. 302 is hypermutable by methyl methanesulfonate (MMS), ethyl methanesulfonate (EMS) and N -methyl- N ′-nitro- N -nitro-soguanidine (MNNG). Both the wild type and 302 excise 7-methylguanine (7-MeG) and 3-methyladenine (3-MeA) from MNNG-methylated DNA but only the wild type excises O 6 -methylguanine ( O 6 -MeG). The mtcA gene product is therefore involved in the initial recognition and/or incision events in excision repair of MTC-mono- and di-adducts and also certain methyl and ethyl products in DNA.
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