Role of tetK-siRNA in inhibition the biofilm formation as a first line of antibiotic resistance by regulation the TetK drug efflux pump in staphylococcus aureus

Abstract

The Staphylococcus aureus (S. aureus) is a common cause of different infections in the community. To determine the effect of siRNA on the mRNA of the tetK gene in S. aureus, 21-23 bp small interfering RNA (siRNA) duplexes were constructed against the mRNA of the tetK gene. The effect of siRNA on tetK mRNA expression was determined using reverse transcription PCR (RT-PCR). To assess changes in biofilm formation in response to siRNA activity, the usual tube technique was adopted. In vitro, tetK-siRNAs inhibited S. aureus mRNA expression and activity. The efficacy of siRNA was determined by comparing the biofilm formation in S. aureus before and after tetK-siRNA was introduced into the bacteria. In this investigation, both modified tetK-siRNA sequence and unmodified tetK-siRNA sequence, were employed. RT-qPCR revealed that both modified tetK-siRNA sequence and unmodified tetK-siRNA sequence significantly suppressed the expression of tetK-mRNA, P = (0.04, and 0.03) respectively at (P < 0.05) comparison with control.