Abstract
The quantitative conversion of sucrose to 3-ketosucrose could be demonstrated with resting cells of Agrobacterium tumefaciens which possess large amounts of invertase showing a strong activity toward 3-ketosucrose. In order to explain the above findings, the sugar transport systems, entry and exit mechnisms, and localization of invertase studies were discussed. The following theory was hypothesized: (1) the locus of invertase in the cells is independent of all sites concerning sugar transport and oxidation of sucrose and (2) the presence of a specific system for 3-ketosucrose efflux reaction is possible.
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