Role of Stem Cells in Regeneration of Myocardium in Experimentally Induced Myocardial Infarction

Abstract

1 Department of Histology and Cell Biology Faculty of Medicine, Benha University, Department of Microbiology and Immunology Military Medical Academy ABSTRACT Introduction: Stem cells have generated a great deal of excitement and promise as a potential source for cell based therapeutic strategies. Primarily owing to their intrinsic ability to self re-new and differentiation into multiple functional cell types. Emberyonic stem cells (EBCs) and bone marrow-derived cells (BMCs) have been studied and dramatic advances have been achieved in their clinical application in ischemic and nonischemic heart failure. Aim of the Work: The present study was done to investigate the healing capacity of autologous bone marrow-derived mesenchymal stem cells (BM-Mscs) and its regenerative role in experimentally induced myocardial infarction. Materials and Methods: The study included two groups of albino rabbits. Group I (n =10) was used a negative control and received no treatment. Group II was used as an experimental group and was divided into subgroup Ha (n=10) used as positive control and subgroup lib (n=10). Animals of subgroup Ila and subgroup lib received a single subcutaneous (s.c.) injection of isoproterenol (150mg/kg) to induce myocardial infarction. Group lib were received undifferentiated mesenchymal stem cells in a dose of (1.41.6 X lOVml. in5ml sterile saline) intravenously (i.v.) 2 weeks after injury. The animals were anaesthetized by ether and killed 2 weeks after transplantation. The regeneration capacity of the engrafted cells was detected by H&E and immunostaining using antivimentin antibodies. Results: Damaged myocardium revealed regeneration denoted by increased content of vimentin proteins in the cytoplasm of the regenerated cardiac myocytes and in the wall of the newly formed small blood vessels. Conclusion: Transplantation of autologous undifferentiated mesenchymal stem cells injected i.v. in rabbits is an effective method of myocardial regeneration in cases of myocardial infarction.