Abstract
背景与目的肺癌是最严重的恶性肿瘤之一，其中非小细胞肺癌发病率在肺癌中居首位。部分患者对顺铂耐受是导致化疗失败的主要原因。SOX4是转录调节因子，在多种肿瘤的发生发展过程中发挥着重要的作用，通过调控β-catenin的表达对Wnt信号通路进行调控。本研究旨在探讨SOX4对非小细胞肺癌细胞A549的顺铂耐药作用的影响。方法体外诱导法建立顺铂耐药的肺癌细胞株A549/DDP，CCK8法检测A549及A549/DDP细胞对顺铂的耐药性。绘制A549与A549/DDP细胞生长曲线。Western blot检测耐药细胞株中SOX4的蛋白表达水平。CCK8法检测敲减SOX4后耐药细胞株A549/DDP对顺铂耐药性。实时定量PCR和免疫印迹法检测敲减SOX4后A549/DPP细胞中β-catenin和Survivin蛋白的表达。结果成功构建非小细胞肺癌顺铂耐药细胞株A549/DDP，其耐药性是亲本细胞的13.7倍，差异有统计学意义（P < 0.001）。生长曲线显示耐药细胞株与亲本细胞增殖速度没有统计学差异（P < 0.05）；与A549细胞相比，耐药细胞A549/DDP细胞中SOX4的表达显著增高（P < 0.001）。敲减SOX4后，A549/DDP细胞的耐药性显著降低；敲减SOX4后，A549/DDP细胞中β-catenin和Survivin的mRNA和蛋白表达水平显著降低。结论SOX4的表达水平影响非小细胞肺癌细胞A549对顺铂的耐药性。
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
