Role of some rhizospheric Pseudomonas on the growth and physiology of broad bean (Vicia faba) under salt stress conditions

Boubaker Idder,Abdelhak Djoudi,Rachid Djibaoui,Hocine Abdelhakim Reguieg Yssaad

doi:10.5586/aa.1794

Abstract

Salt stress affects the development and growth of plants in various ways as a result of its effect on water relationships, photosynthesis, and nutrient absorption by physiological and biochemical processes. Consequently, several researchers have increasingly studied the effect of plant growth promoting bacteria (PGPR) as promoters and enhancers under saline environment. The main goals of this study were to examine the manifested response of the broad bean plant under saline conditions and to evaluate the role of some Pseudomonas isolates in improving plant tolerance to salt stress. Three Pseudomonas strains were isolated (P1 and P7 from a saline soil and P15 from a vineyard soil). These isolates were screened by salinity and used as inoculums in Vicia faba plants (OTONO variety) irrigated with two saline solutions (NaCl; 100 and 150 mM L−1) and one without salinity. The results show that salinity decreased the fresh weight, total chlorophyll content, and the Na+/K+ ratio, but it increased proline accumulation in inoculated and noninoculated plants. The inoculation of V. faba plants with P1, P7, and P15 strains significantly increased the production of fresh biomass in the presence and absence of salt stress, and positively affected the accumulation of proline and the Na+/K+ ratio. The inoculation with bacterial strains increased the total chlorophyll content in plants at all salt treatment levels, especially the P1 strain that showed a significant effect.

Highlights

Salinity is considered to be one of the main abiotic stresses in the Sahara region because it reduces the area of exploitable land by 1% to 2% yearly [1]
This study aimed to investigate the role of rhizobacterial Pseudomonas inoculation in the clearance of salt stress effects on the growth and physiology of V. faba
The results showed that the 100 and 150 mM L−1 saline treatments induced a regression in the fresh weight of V. faba plants inoculated with P1, P7, and P15 and the noninoculated plants

Summary

Introduction

Salinity is considered to be one of the main abiotic stresses in the Sahara region because it reduces the area of exploitable land by 1% to 2% yearly [1]. Over 800 million hectares of land are affected by salinity, which could drastically reduce agricultural productivity [2]. Salinity reduces nutrient absorption by plants, phosphate uptake [3]. Saline stress affects several biochemical and metabolic processes in plants, including protein synthesis, photosynthesis, and lipid metabolism, growth and yields are reduced [5,6]. The accumulation of ions, such as sodium chloride, can alter many physiological activities [7], decrease productivity, and cause plant death [2]

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