Abstract
DECOLORIZATION of textile reactive azo dyes, blue and yellow, by 15 bacterial isolates collected from Egyptian arid soil was investigated. Of these, S8 isolate revealed a highest decolorization performance. Here, we identified S8 isolate on a molecular level based on type I chaperonin universal target sequence (cpn60). The most similar DNA sequence to the S8 DNA sequence was for Klebsiella oxytoca partial CDS (AB008147.1) coding for GroES and GroEL chaperon homologues (acronyms of cpn60) with 88% DNA sequence similarity. The impact of numerous exterior parameters to improve the decolorization abilities of this isolate was studied. A maximum decolorization activity occurred at a medium containing glucose, soybean husk at a C/N ratio of 12:1 supplemented with dye concentration of 100mg/L and amended with 3% (v/v) inoculum. Incubation for 4 days at 35°C±2 with shaking at 150rpm reached the decolorization activity to 89.35 and 78.23% for blue and yellow dyes, respectively. The ascending levels of bacterial enzymes like azo-reductase, phenol red manganese peroxidase and ascorbate oxidase indicated their prominent roles in dye degradation.
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