Role of some biomarkers in placental malaria in women living in Yaoundé, Cameroon

Abstract

Despite intensive research on the immunpathology of placental malaria (PM), the role of some β-chemokines known to attract inflammatory cells is less known. This study sought to determine the role of CXCL-10, IL-10, IL-19, IL-17A and IL-23 in placental malaria in women at delivery. Between 2010 and 2011, paired peripheral and placental blood specimens were collected from 139 Cameroonian women at delivery. Differential white blood cell counts and malaria parasitaemia were determined microscopically while the accumulation of parasites in the placenta was investigated through histological studies. Plasma levels of CXCL-10, IL-10, IL-17A, IL-19 and Il-23 were determined by ELISA. The cytokines IL-10, IL-17A and IL-23 were predominant in peripheral plasma from both infected and non-infected women. While IL-10 associated negatively with parity, IL-23 showed a positive correlation (p<0.05). The production of CXCL-10 was independent of parity and higher in placental plasma. There was an association between the plasma levels of IL-10 and CXCL-10 with malaria parasitaemia in the placenta impression smears, placental and peripheral blood and the presence of malaria pigments in the placenta tissue. Leukocyte accumulation into the intervillous space correlated positively with plasma levels of placental IL-17A (p<0.001). Parity also associated with peripheral IL-17A (p=0.016). The peripheral and placental plasma levels of CXCL-10 and IL-10 also correlated positively with monocyte counts (p=0.011–0.042) while a negative correlation was found with lymphocyte counts (p=0.017 to <0.001) of the impression smear. However, the levels of IL-10 in both peripheral and placental plasma and CXCL-10 in placental plasma only, were higher in low birth weight baby. With regards to IL-17A, its placental plasma level correlated positively with lymphocyte counts of placental blood (p=0.045). During PM, CXCL-10 might attract monocytes and lymphocytes into the placenta where they produce inflammatory cytokines such as IL-10 and IL-17A to modulate the disease, which affect baby weight.