Role of Renal Nerves in Hemodynamic and Natriuretic Responses to Saline in Rabbits with Impaired Baroreflex Sensitivity

Abstract

The hemodynamic and urinary Na+ excretory response to a 2.5 -fold increase in NaCl by iv infusion were assessed in conscious male rabbits with either high (BShi, salt-insensitive) or low (BSlo, salt-sensitive) cardiac baroreflex sensitivity, before, and 11–14 days after bilateral renal denervatiion. Effective renal plasma flow (ERPF) and proximal tubular Na+ reabsorption were measured by para-amino-hippumte (PAR) and Li+ clearances, respectively, before and after NaCl infused for 2 hr at a rate of 0.11 mL/kg/min. Intact BShi rabbits, showed a significant natriuresis within 30 min which was associated with an increase in ERPF and inhibition of proximal tubular reabsorption. The Na+ excretion rate was much slower in BSlo rabbits, while ERPF and proximal tubular reabsorption remained unchanged. Renal denervation reduced MAP, in-creased basal ERPF, Na+ and Li+ excretion in both groups, and abolished the difference in the renal hemodynamic re-sponse and Li+ excretion to increased NaCl, but not that in the rate of Na+ excretion. The data suggest that BSlo rabbits do not increase their ERPF and Li+ in response to saline because of an inability to bring about an inhibition of renal sympathetic nerve activity. This could be due to an impairment in the sensitivity of their cardiopulmonary baroreceptors. The difference in the rate of natriuresis in the two groups of rabbits which remained after renal denervation could involve an additional hormonal or a local renal mechanism.