Abstract
Top of pageAbstract RBC contain most AA at concentrations as high or higher than plasma. Yet their role in AA interorgan transport is controversial, since AA uptake by RBC is very slow in vitro. We therefore used 4-hr primed, continuous intravenous infusions of L-[13C]leucine, [15N]glycine, and L-[15N]alanine to assess AA exchange between plasma and RBC in vivo. Stable isotope enrichments and AA levels were measured by gas chromatography-mass spectrometry in both plasma and whole blood, and calculated (using hematocrit) for RBC. Results were as follows (mean of 5 subjects ± S.D.): We conclude that the exchange of leucine between plasma and RBC in vivo is much faster than that of alanine and glycine. These data suggest that the role of RBC in AA interorgan transport differs widely from one class of AA to the other.
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