Role of RASSF1-A gene in early detection of colorectal carcinogenesis

Abstract

Background: Colorectal cancer (CRC) is a malignant neoplasm originating from the un-controlled cell proliferations in epithelial cells lining of rectum, colon, or appendix. RASSFs wereconcerned in tumourigenesis and numerous members of family are presently supposed to be tumour suppressor, Aim and objectives: Estimate- function of RASSF1-A methylated gene in theCRC pathogenesis, detect RASSF1-A methylated gene cutoff value for diagnosis and using RASSF1-A gene as abio-marker to detect recurrence after surgery, Subjects and methods: This is a prospective observational case-control researchperformed on 50 cases diagnosed as CRC by endoscopy and histopathology and 50 control persons at Aswan university hospital and internal medicine department., Results: there is high significant relation between RASSF 1A and development of colorectal-cancer, Conclusion: Genetical mutations in the Kras genes and epigenetic modifications in RASSF1-A, FHIT and MGMT genes in sporadic CRC are accompanying with the generalprogress of the disorder and can be utilized as diagnostical or prognostically biomarkers in this collection of tumours.